LAMP2 Antikörper (FITC)

Produktnummer ABIN7835618

Der FITC-konjugierte Ratte Monoklonal anti-LAMP2 Antikörper (Klon ABL-93) (ABIN7835618) detektiert spezifisch LAMP2 in FACS und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für LAMP2 Antikörper (FITC) (ABIN7835618)

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser LAMP2 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: ABL-93

Produktdetails anti-LAMP2 Antikörper (FITC)

Verwendungszweck: Rat monoclonal antibody FITC labled to CD107b

Spezifität: Recognizes mouse CD107b

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Membrane glycoproteins from C57BL/6 mouse peritoneal exudate cells

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu LAMP2

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Andere Bezeichnung: LAMP2

Hintergrund: Lysosome-associated membrane glycoprotein 2, LAMP-2, Lysosome-associated membrane protein 2, CD107 antigen-like family member B, CD107b

Gen-ID: 16784

Pathways: Autophagie