LAMP1 Antikörper (N-Term) (Alexa Fluor 647)

Produktnummer ABIN7835604

Dieser Ratte Monoklonal Antikörper detektiert spezifisch LAMP1 in FACS und IF. Es zeigt Reaktivität gegenüber Proben von Maus.

Kurzübersicht für LAMP1 Antikörper (N-Term) (Alexa Fluor 647) (ABIN7835604)

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser LAMP1 Antikörper ist konjugiert mit Alexa Fluor 647

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 1D4B

Produktdetails anti-LAMP1 Antikörper (Alexa Fluor 647)

Bindungsspezifität: N-Term

Verwendungszweck: Rat monoclonal antibody AF647 labled to CD107a

Spezifität: Recognizes mouse CD107a

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: This monoclonal antibody was raised against NIH/3T3 mouse embryo fobroblast tissue culture cell membranes. It has been mapped to the N-terminus of LAMP-1.

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu LAMP1

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Andere Bezeichnung: LAMP1

Hintergrund: Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, CD107a

Gen-ID: 16783

Pathways: Autophagie