VCAM1 Antikörper (FITC)

Produktnummer ABIN7835596

Der Ratte Monoklonal anti-VCAM1 Antikörper wird verwendet zum Nachweis von VCAM1 in Proben von Maus. Er wurde validiert für FACS und IF.

Kurzübersicht für VCAM1 Antikörper (FITC) (ABIN7835596)

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser VCAM1 Antikörper ist konjugiert mit FITC

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: M-K-2

Produktdetails anti-VCAM1 Antikörper (FITC)

Verwendungszweck: Rat monoclonal antibody FITC labled to CD106

Spezifität: Recognizes mouse CD106

Aufreinigung: The antibody was purified by affinity chromatography.

Immunogen: Mouse preadipose cell line PA6

Anwendungsinformationen

Applikationshinweise: Flow: 10 μL / assay

Protokoll: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Shipped and store at 4°C for one year. Do not freeze.

Details zu VCAM1

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Andere Bezeichnung: VCAM1

Hintergrund: L1CAM, Vascular cell adhesion protein 1, V-CAM 1, VCAM-1, INCAM-100, CD106

Gen-ID: 22329

Pathways: Carbohydrate Homeostasis