RBCL Antikörper (HRP)

Produktnummer ABIN7833500

Der HRP-konjugierte Kaninchen Polyklonal anti-RBCL Antikörper (ABIN7833500) detektiert spezifisch RBCL in ELISA und WB. Dieser Antikörper reagiert spezifisch mit Proben aus Arabidopsis thaliana, Soybean, Alfalfa, Synechococcus sp., Spinach, Pea, Kochia prostrata, Physcomytrium Patens, Phaeodactylum tricornutum, Petunia hybrida, Panicum virgatum, Nicotiana tabacum, Nicotiana benthamiana, Micromonas pusilla, Methylacidiphilum fumariolicum, Liquidambar formosana, Lathyrus sativus, Porosira glacialisPorphyra (Red Algae), Karenia brevis, Kandelia candel, Hypnum cupressiforme, Hevea, Heterosigma akashiwo, Guzmania hybrid, Gonyaulax polyedra, Galdieria sulphuraria, Vitis vinifera, Tradescantia spathacea, Tomato, Verbascum lychnitis, Tisochrysis lutea, Thermosynechococcus elongatus, Thalassiosira punctigera, Thalassiosira pseudonana, Symbiodinium, Stanleya pinnata, Polytrichum formosum, Schima superba, Saccharum, Robinia pseudoacacia, Ricinus communis, Rhytidiadelphus squarrosus, Rape, Quercus ilex, Prochlorococcus, Triticum aestivum, Chlamydomonas raudensis, Apple, Artemisia annua, Atrichum undulatum, Attheya longicornis, Baculogypsina sphaerulata, Barbados Nut (Jatropha curcas), Gerste, Beet, Begonia, Bienertia sinuspersici, Cannabis sativa L., Celery, Chaetoceros neogracilis, Chenopodium quinoa, Chickpea, Cylindrospermopsis raciborskii, Fucus vesiculosus, Fraxinus mandshurica, Fortunella margarita, Ficus, Euglena gracilis, Emiliania huxleyi, Diatom (Skeletonema), Cynara cardunculus, Chlamydomonas reinhardtii, Cyanophora paradoxa, Coscinodiscus concinnus, Colobanthus quitensis, Chlorococcum sp. 'dorsiventrale', Chlorella vulgaris, Chlorella sorokiniana und Agrostis stolonifera.

Kurzübersicht für RBCL Antikörper (HRP) (ABIN7833500)

Target: RBCL

Reaktivität: Arabidopsis thaliana, Soybean, Alfalfa, Synechococcus sp., Spinach, Pea, Kochia prostrata, Physcomytrium Patens, Phaeodactylum tricornutum, Petunia hybrida, Panicum virgatum, Nicotiana tabacum, Nicotiana benthamiana, Micromonas pusilla, Methylacidiphilum fumariolicum, Liquidambar formosana, Lathyrus sativus, Porosira glacialisPorphyra (Red Algae), Karenia brevis, Kandelia candel, Hypnum cupressiforme, Hevea, Heterosigma akashiwo, Guzmania hybrid, Gonyaulax polyedra, Galdieria sulphuraria, Vitis vinifera, Tradescantia spathacea, Tomato, Verbascum lychnitis, Tisochrysis lutea, Thermosynechococcus elongatus, Thalassiosira punctigera, Thalassiosira pseudonana, Symbiodinium, Stanleya pinnata, Polytrichum formosum, Schima superba, Saccharum, Robinia pseudoacacia, Ricinus communis, Rhytidiadelphus squarrosus, Rape, Quercus ilex, Prochlorococcus, Triticum aestivum, Chlamydomonas raudensis, Apple, Artemisia annua, Atrichum undulatum, Attheya longicornis, Baculogypsina sphaerulata, Barbados Nut (Jatropha curcas), Gerste, Beet, Begonia, Bienertia sinuspersici, Cannabis sativa L., Celery, Chaetoceros neogracilis, Chenopodium quinoa, Chickpea, Cylindrospermopsis raciborskii, Fucus vesiculosus, Fraxinus mandshurica, Fortunella margarita , Ficus, Euglena gracilis, Emiliania huxleyi, Diatom (Skeletonema), Cynara cardunculus, Chlamydomonas reinhardtii, Cyanophora paradoxa, Coscinodiscus concinnus, Colobanthus quitensis, Chlorococcum sp. 'dorsiventrale', Chlorella vulgaris, Chlorella sorokiniana, Agrostis stolonifera

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Western Blotting (WB)

Produktdetails

Verwendungszweck: Anti-RbcL | Rubisco large subunit, form I, HRP-conjugated

Kreuzreaktivität (Details): Agostis stolonifera cv. Penncross, Arabidopsis thaliana, Apium graveolens, Artemisia annua, Atrichum undulatum, Attheya longicornis, Baculogypsina sphaerulata (benthic foraminifer), Beta vulgaris, Begonia sp., Bienertia sinuspersici, Brassica napus, Kandelia candel, Cannabis sativa L., Chaetoceros furcellatus, Chlorococcum dorsiventrale, Colobanthus quitensis, Cicer arietinum, Chenopodium quinoa, Chlamydomonas raudensis, Chlamydomonas reinhardtii, Colobanthus quitensis Kunt Bartl, Chlorella sorokiniana, Chlorella vulgaris, Coscinodiscus concinnus, Cyanophora paradoxa, Cylindrospermopsis raciborskii CS-505, Cynara cardunculus, Emiliana huxleyi, Euglena gracilis, Ficus carica, Fortunella margarita Swingle, Fraxinus mandshurica, Fucus vesiculosus, Gladieria sulphuraria, Glycine max, Gonyaulax polyedra, Guzmania hybrid, Heterosigma akashiwo, Hevea, Hordeum vulgare, Hypnum cupressiforme, Jatropha curcas, Karenia brevis (C.C.Davis) s) G.Hansen & U.Moestrup (Wilson isolate), Kochia prostrata, Lathyrus sativus, Liquidambar formosana, Malus domestica, Medicago truncatula, Micromonas pusila, Nicotiana benthamiana, Nicotiana tabacum, Panicum virgatum, Petunia hybrida cv. Mitchell, Phaeodactylum tricornutum, Physcomitrium patens, Pisum sativum, olytrichum formosum, Porosira glacialis, Porphyra sp., Ricinus communis, Robinia pseudoacacia, Rhytidiadelphus squarrosus, Saccharum sp., Schima superba, Skeletonema costatum (diatom), Skeletonema marinoi (diatom), Solanum lycopersicum, Spinacia oleracea, lichens, Stanleya pinnata, Symbiodinium sp., Synechococcus PCC 7942, Synechococcus elongatus UTEX 2973, Rhoeo discolor, Thalassiosira pseudonana, Thermosynechococcus elongatus, Triticum aestivum, Prochlorococcus sp. (surface and deep water ecotype), Triticum aestivum, dinoflagellate endosymbionts (genus Symbiodinium), extreme acidophilic verrucomicrobial methanotroph Methylacidiphilum fumariolicum strain SolV, Thalassiosira punctigera, Tisochrysis lutea, Verbascum lychnitis, Vitis vinifera, Quercus ilex
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Alpha proteobacteria,Algae (brown,red) including Galdieria sulphuraria,Dicots,Benincasa hispida,Kalanchoe fedtschenkoi, Beta-proteobacteria,Conifers,Cryptomonads,Cyanobacteria (prochlorophytes),Gamma-proeobacteria,Liverworts,Manihot esculenta,Marchantia polymorpha,Monocots,Mosses,Suaeda glauca,Welwitschia, Nannochloropsis sp.,Picochlorum sp.,Porphyridium purpureum,Zea mays,Zosteria marinaFor detection in Rhodospirillaceae use product AS15 2955

Aufreinigung: Immunogen affinity purified serum

Immunogen: KLH-conjugated synthetic peptide conserved across all known plant

Anwendungsinformationen

Applikationshinweise: 1 : 5000 (ELISA), 1 : 10 000-25 000 (WB)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Immunogen affinity purified serum in PBS pH 7.4, conjugated to HRP.

Handhabung: A preservative may be added for long time storage

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.

Haltbarkeit: 12-24 months

Antigendetails

Target: RBCL

Hintergrund:

Background: Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.This antibody is directly conjugated to HRP.

Additional information: Anti-RbcL can be used as a cellular [compartment marker] of plastid stroma (cytoplasm in cyanobacteria) and detects RbcL protein from 31.25 fmoles. As both forms (I and II) are detected it is suitable for work with samples from Dinoflagellates, Haptophytes and Ochrophytes (diatoms, Raphidophytes, brown algae) as well as higher plants. This antibody together with Rubisco protein standard is very suitable to quantify Rubisco in plant and algal samples.

Molekulargewicht: 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 (Chlamydomonas reinhardtii)

Gen-ID: 844754

UniProt: O03042, P05698, P0C510, P00877