RBCL (Isoform 1) Antikörper

Produktnummer ABIN93524

Es sind 3+ Publikationen für dieses Produkt verfügbar. Der Huhn Polyklonal anti-RBCL Antikörper wird verwendet zum Nachweis von RBCL in Proben von Arabidopsis thaliana, Pea, Alfalfa, Spinach, Synechococcus sp., Ulmus, Thiobacillus, Spartina alterniflora, Potato, Pinus strobus, Microcystis aeruginosa, Lobaria pulmonaria und Diatom (Fragilariopsis). Er wurde validiert für WB, EM, IF und IL.

Kurzübersicht für RBCL (Isoform 1) Antikörper (ABIN93524)

Target: RBCL

Reaktivität: Arabidopsis thaliana, Pea, Alfalfa, Spinach, Synechococcus sp., Ulmus, Thiobacillus, Spartina alterniflora, Potato, Pinus strobus, Microcystis aeruginosa, Lobaria pulmonaria, Diatom (Fragilariopsis)

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Electron Microscopy (EM), Immunofluorescence (IF), Immunolocalization (IL)

Produktdetails

Bindungsspezifität: Isoform 1

Verwendungszweck: Anti-RbcL | Rubisco large subunit, form I (chicken)

Kreuzreaktivität (Details): Arabidopsis thaliana, Fragilariopsis cylindrus, Lobaria pulmonaria, Medicago sativa, mixed phytoplankton, Microcystis aeruginosa PCC7806, Pinus strobus, Pisum sativum, Solanum tuberosum, Spartina alterniflora, Spinacia oleracea, Synechococcus sp. PCC7842, Thiobacillus sp. Ulmus sp.
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Homologie: Algae,Dicots,Conifers,Liverworts,Mosses,Prochlorophytes,Welwitschia

Aufreinigung: Purified, total IgY

Immunogen: KLH-conjugated synthetic peptide derived from all known plant

Anwendungsinformationen

Applikationshinweise: (IL) tested on a grass species, formaldehyde-fixed and paraffin-embedded tissue following the protocol from Gonzalez et al, (1998) Plant Physiol, V, 116, 1 : 10 000-1 : 20 000, 2 μg of total cellular protein, (WB)

Kommentare:

This antibody detects RbcL protein from 102.6 fmoles and has been used as a control to ensure adequate permeabilization and fixation of toxic cyanobacterial cells in immunolabeling experiments (method based on: Orellana & Perry (1995) J Phycol 31: 785-794).Antibody has been used in immunolabelling of intact cyanobacterial cells fixed with ethanol using a secondary anti-IgY antibody conjugated with a fluorochrome.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 16 µg/µL

Buffer: Purified, total IgY (chicken egg yolk immunoglobulin) in PBS pH 8. Contains 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: 4 °C

Informationen zur Lagerung: Store at 4°C, make aliquots to avoid working with a stock. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Referenzen

Abdel-Ghany, Pilon: "MicroRNA-mediated systemic down-regulation of copper protein expression in response to low copper availability in Arabidopsis." in: The Journal of biological chemistry, Vol. 283, Issue 23, pp. 15932-45, (2008) (PubMed).

Ensminger, Schmidt, Lloyd: "Soil temperature and intermittent frost modulate the rate of recovery of photosynthesis in Scots pine under simulated spring conditions." in: The New phytologist, Vol. 177, Issue 2, pp. 428-42, (2008) (PubMed).

Menon, Dou, Heinhorst, Shively, Cannon: "Halothiobacillus neapolitanus carboxysomes sequester heterologous and chimeric RubisCO species." in: PLoS ONE, Vol. 3, Issue 10, pp. e3570, (2008) (PubMed).

Antigendetails

Target: RBCL

Hintergrund:

Background: Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthesis. It is one of the most abundant proteins on Earth and its homology has been demonstrated from purple bacteria to flowering plants.

Additional information: Peptide target used to elicit this antibody is not conserved in type II Rubisco found in dinoflagellates and some photosynthetic bacteria

Molekulargewicht: 52.7 kDa (Arabidopsis thaliana), 52.5 kDa (cyanobacteria), 52.3 kDa (Chlamydomonas reinhardtii)

Gen-ID: 844754

UniProt: O03042, Q3ALL1