LC3B Antikörper

Produktnummer ABIN7833424

Der Kaninchen Polyklonal anti-LC3B Antikörper wird verwendet zum Nachweis von LC3B in Proben von Mais (Zea mays), Tomato, Poplar (Populus), Nicotiana benthamiana, Haematococcus lacustris, Gossypim hirsutum, Chlorococcum sp. 'dorsiventrale', Chlamydomonas reinhardtii, Arabidopsis thaliana und Aponogeton madagascariensis. Er wurde validiert für WB und IL.

Kurzübersicht für LC3B Antikörper (ABIN7833424)

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Reaktivität: Mais (Zea mays), Tomato, Poplar (Populus), Nicotiana benthamiana, Haematococcus lacustris, Gossypim hirsutum, Chlorococcum sp. 'dorsiventrale', Chlamydomonas reinhardtii, Arabidopsis thaliana, Aponogeton madagascariensis

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LC3B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunolocalization (IL)

Produktdetails anti-LC3B Antikörper

Verwendungszweck: Anti-ATG8 | Autophagy-related protein

Kreuzreaktivität (Details): Arabidopsis thaliana, Aponogeton madagascariensis, Chlamydononas reinhardtii, Chlorococcum dorsiventrale, Gossypim hirsutum, Haematococcus lacustris, Nicotiana benthamiana, Populus trichocarpa, Solanum lycopersicum, Zea mays
Not reactive in: Cuscuta chinensis, Symbiodinium sp.

Homologie: Ananas comosus,Brassica napus,Hordeum vulgare,Micromonas sp.,Nelumbo nucifera,Oryza sativa,Panicum hallii,Parachlorella kessleri,Phoenix dactylifera,Pyrus x bretschneideri,Physcomitrium patens,Pinus sitchensis,Solanum tuberosum,Triticum aestivum,Volvox carteri

Aufreinigung: Serum

Immunogen: Fragment of recombinant ATG8 from Chlamydomonas reinhardtii

Anwendungsinformationen

Applikationshinweise: 1 : 1000 (IL), 1 : 1000-1 : 2000 (WB)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Details zu LC3B

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Andere Bezeichnung: ATG8

Hintergrund:

Background: ATG8 (Autophagy-related protein 8) is involved in degradation and recycling of intracellular components in a process of autophagy. ATG8 is a molecular autophagy marker in Chlamydomonas reinhardtii (Pérez-Pérez et al. 2010, Plant Physiol. 152: 1874-88).

Additional information: This product can be sold containing ProClin if requested.This antibody is recognizing 1 ng of recombinant CrATG8. Antigen used to elicit this antibody is conserved from 70-80 % in following ATG protein from Arabidopsis thaliana: ATG8a UniProt: Q8LEM4 ATG8B UniProt: Q9XEB5 ATG8c UniProt: Q8S927 ATG8d UniProt: Q9SL04, ATG8e UniProt: Q8S926 ATG8f UniProt: Q8VYK7 and conserved below 70 % in: ATG8g UniProt: Q9LZZ9 ATG8h Uniprot: Q8S92This antibody does not recognize all isoforms into the same degree.

Molekulargewicht: 15.2 | 15 kDa

Gen-ID: 5724800

UniProt: A8JB85

Pathways: Autophagie