LexA Antikörper

Produktnummer ABIN7833406

Der Kaninchen Polyklonal anti-LexA Antikörper wird verwendet zum Nachweis von LexA in Proben von E. coli. Er wurde validiert für WB, ChIP und IL.

Kurzübersicht für LexA Antikörper (ABIN7833406)

Target: LexA

Reaktivität: E. coli

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Immunolocalization (IL)

Produktdetails

Verwendungszweck: Anti-LexA | LexA repressor

Kreuzreaktivität (Details): Escherichia coli

Immunogen: Full-size highly-purified recombinant LexA protein

Anwendungsinformationen

Applikationshinweise: 1: 1000 - 1: 3000 (WB)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Serum. Contains 0.05 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Antigendetails

Target: LexA

Hintergrund:

Background: LexA represor binds specifically to the SOS-box sequence and represses the genes belonging to the SOS regulation. In response to DNA damage, RecA protein is activated by ss-DNA accumulated in the damaged cells and promotes autocleavage of LexA repressor by its coprotease activity. As a result, DNA repair genes and error prone polymerases are induced, and DNA damage is repaired and mutation is induced (1). The lexA gene is used for yeast two-hybrid experiments as a bait to identify the protein-protein interaction in vivo.Alternative names: exrA, spr, tsl, umuA

Additional information: This product can be used in:Studies of SOS regulation in E.coliDetection of bait constructs in yeast two-hybrid systemImmunolocalization of LexA fusion proteins (fixed with 4 % formaldehyde)Immunoprecipiation and ChIP

Molekulargewicht: 22.3 | 23 kDa

Gen-ID: 948544

UniProt: P0A7C2