eGFP Antikörper

Produktnummer ABIN7833234

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch eGFP in WB und ICC. Es zeigt Reaktivität gegenüber Proben von Aequorea victoria.

Kurzübersicht für eGFP Antikörper (ABIN7833234)

Target: eGFP (Enhanced Green Fluorescent Protein (eGFP))

Reaktivität: Aequorea victoria

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser eGFP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC)

Produktdetails anti-eGFP Antikörper

Verwendungszweck: Anti-EGFP | Enhanced Green Fluorescence Protein

Kreuzreaktivität (Details): GFP, EGFP
Not reactive in: mCherry

Aufreinigung: Immunogen affinity purified

Immunogen: Recombinant GFP from Aequorea coerulescens (belt jellyfish) overexpressed and purified from E.coli

Anwendungsinformationen

Applikationshinweise: 1:2000 - 1:5000 (ICC), 1:1000 - 1:5000 (WB)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 μL of sterile water, ultrapure water

Buffer: Immunogen affinity purified in PBS pH 7.2-7.6, 0.1 % trehalose, 5 mM sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store lypholized antibody at 4 °C. After reconstitution keep aliquots at -20°C for a higher stability. Avoid repetitive freeze/thaw cycles. Centrifuge briefly to remove any insoluble material.Expiry date: 12 months after purchase if unopened

Haltbarkeit: 12 months

Details zu eGFP

Target: eGFP (Enhanced Green Fluorescent Protein (eGFP))

Andere Bezeichnung: EGFP

Hintergrund: EGFP is an Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Xaa-N and Gly-(N+2), and oxidation of Tyr-(N+1) to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

Molekulargewicht: 27 kDa (for GFP without fusion partner)