eGFP Antikörper (AA 1-239)

Produktnummer ABIN7596110

Der Maus Monoklonal anti-eGFP Antikörper (Klon AT1D9) (ABIN7596110) detektiert spezifisch eGFP in WB und ELISA. Dieser Antikörper reagiert spezifisch mit Proben aus Aequorea victoria.

Kurzübersicht für eGFP Antikörper (AA 1-239) (ABIN7596110)

Target: eGFP (Enhanced Green Fluorescent Protein (eGFP))

Reaktivität: Aequorea victoria

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser eGFP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Klon: AT1D9

Produktdetails anti-eGFP Antikörper

Bindungsspezifität: AA 1-239

Verwendungszweck: EGFP antibody

Aufreinigung: protein-A affinity chromatography

Immunogen: Recombinant EGFP(1-239aa) purified from E. coli

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycero

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Details zu eGFP

Target: eGFP (Enhanced Green Fluorescent Protein (eGFP))

Andere Bezeichnung: EGFP

Hintergrund: Green fluorescent protein (GFP) here refers to the protein first purified from jellyfish Aequorea victoria, though many other organisms have similar proteins. It is a 26.9 kDa protein (composed of 238 a.a. residues) that shows green fluorescence in shortwave light (blue to ultraviolet). Despite of wild-type GFP, many mutants of GFP have been engineered for wider usage in research. Enhanced GFP (eGFP) has S65T and F64L mutations, which make GFP show increased fluorescence and fold more efficiently under 37 degrees C, respectively. eGFP allows the use of GFP in mammalian cells. In A. Victoria, GFP plays roles as an energy transfer acceptor. It has long been used in cell and molecular biology as a reporter of gene expression. GFP can also been applied as a molecular thermometer to measure temperature accurately in fluids.