Rekombinanter ube3a Antikörper

Produktnummer ABIN7828657

Dieser Kaninchen Monoklonal Antikörper detektiert spezifisch ube3a in WB. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Rekombinanter ube3a Antikörper (ABIN7828657)

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser ube3a Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: A758

Produktdetails anti-ube3a Antikörper

Verwendungszweck: Recombinant UBE3A Monoclonal Antibody

Aufreinigung: Protein A purified

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise: WB 1:5000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

Haltbarkeit: 12 months

Details zu ube3a

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Andere Bezeichnung: UBE3A

Hintergrund: UBE3A,ANCR,AS,E6-AP,EPVE6AP,HPVE6A,E6AP,ubiquitin protein ligase E3A,UBE3A, also commonly referred to as E6AP (E6 Associated Protein), is an E3 ubiquitin protein ligase and founding member of the HECT (Homologous to the E6 Carboxyl Terminus) family of E3 ligases. UBE3A has been shown to be hijacked by the oncogenic E6 protein of high-risk human papillomaviruses (HPV16 and HPV18) that causes the ubiquitination activity of UBE3A to be inappropriately directed toward several specific cellular proteins, the most notable of which, with respect to carcinogenesis, is p53. Although the DNA-repair enzyme, HHR23A (human homolog A of Rad23), was the first described E6-independent substrate of UBE3A, very few E6-independent targets of UBE3A have been identified. This continues to be an active area of research, particularly because mutations or disruption in expression of UBE3A in the brain are the cause of Angelman syndrome (AS), a severe form of mental retardation. Although UBE3A is expressed in most human tissues from both parental alleles, it is expressed from the maternal allele in subregions of the brain, with the paternal allele being epigenetically silenced. AS is caused by disruptions in expression of the materal UBE3A allele, generally by large chromosomal deletion, but also by point mutations within the UBE3A coding sequence. This strongly suggests that lack of ubiquitination of one or more UBE3A substrates in neuronal tissue is responsible for the AS phenotype. Indeed, a recent study identified several new neuronal substrates of UBE3A including Arc and Ephexin-5. The immediate early gene Arc (activity-regulated cytoskeleton-associated protein) is rapidly upregulated after robust neuronal stimulation and promotes internalization of AMPA-type glutamate receptors (AMPARs), resulting in reduction in synaptic strength. UBE3A ubiquitinates Arc and promotes its degradation by the 26S proteasome, thus preventing AMPAR internalization. Disruption in neuronal UBE3A function leads to an increase in Arc expression and a decrease in AMPARs at excitatory synapses, which may contribute to the neurological symptoms of AS. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

Molekulargewicht:

Calculated MW: 101 kDa

Observed MW: 95 kDa The actual band is not consistent with the expectation.

UniProt: Q05086

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway