Der Kaninchen Monoklonal anti-Topo IIalpha Antikörper wird verwendet zum Nachweis von Topo IIalpha in Proben von Human und Maus. Er wurde validiert für WB, IHC und ICC.
Reaktivität: Human
WB, ELISA, IHC (p)
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
ICC,1100 to 1500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.,IHC,1100 to 1500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.,WB,11,000
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Phosphate Buffered Saline (PBS) with 0.1 % BSA and 0.09 % Sodium Azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C
Informationen zur Lagerung
2 - 8 °C
Haltbarkeit
12 months
Target
Topo IIalpha
Andere Bezeichnung
Topo II Alpha
Hintergrund
Topoisomerases regulate topological states of DNA and are divided into type I and type II enzymes. Type II topoisomerases make double stranded breaks in DNA and allow the passage of another duplex DNA across the break to achieve unknotting of entangled DNA. Topo II Alpha is one of two described isoforms that are likely products of a gene duplication event. TopoII Alpha is also described to be involved in chromosome condensation, chromatid separation, and the relief of supercoil stress.