Topo IIalpha (C-Term) Antikörper

Produktnummer ABIN7454588

Der Kaninchen Polyklonal anti-Topo IIalpha Antikörper (ABIN7454588) detektiert spezifisch Topo IIalpha in WB, IHC (fp) und IP. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Topo IIalpha (C-Term) Antikörper (ABIN7454588)

Target: Topo IIalpha

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP)

Produktdetails

Bindungsspezifität: C-Term

Verwendungszweck: Rabbit anti-Topo II Alpha Antibody, Affinity Purified

Aufreinigung: Affinity Purified

Immunogen: between AA 1500 and C-term

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

IHC: 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:10,000 - 1:30,000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Haltbarkeit: 12 months

Antigendetails

Target: Topo IIalpha

Andere Bezeichnung: Topo II Alpha

Hintergrund: Background: Topoisomerases regulate topological states of DNA and are divided into type I and type II enzymes. Type II topoisomerases make double stranded breaks in DNA and allow the passage of another duplex DNA across the break to achieve unknotting of entangled DNA. Topo II Alpha is one of two described isoforms that are likely products of a gene duplication event. TopoII Alpha is also described to be involved in chromosome condensation, chromatid separation, and the relief of supercoil stress.

Gen-ID: 7153

NCBI Accession: NP_001058

UniProt: P11388