Rekombinanter CD3 epsilon Antikörper

Produktnummer ABIN7795320

Der Maus Monoklonal Anti-CD3 epsilon Antikörper wurde für FACS validiert. Er ist geeignet, CD3 epsilon in Proben von Maus zu detektieren.

Kurzübersicht für Rekombinanter CD3 epsilon Antikörper (ABIN7795320)

Target: CD3 epsilon (CD3E)

Antikörpertyp: Recombinant Antibody

Reaktivität: Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD3 epsilon Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS)

Klon: 145-2C11

Produktdetails anti-CD3 epsilon Antikörper

Expressionssystem: Mammalian cells

Verwendungszweck: Recombinant Anti-mouse CD3e Monoclonal Antibody (Clone: 145-2C11), Mouse IgG2a Kappa

Produktmerkmale: The antibody binds to CD3 epsilon of the T cell receptor-CD3 complex found only on mature T cells and medullary thymocytes. An interaction between T cells, the antibody, and monocytes causes T-cell activation (mitogenesis) in vitro. On the other hand, the antibody blocks the unique association between the antigen receptor and CD3, and both the generation and function of cytotoxic T cells. After an initial dose of the antibody, T cells disappear from the circulation within minutes to hours. During treatment with the antibody, T cells bearing the usual array of surface molecules (CD2, CD4, and CD8) reappear in the peripheral blood circulation but these cells are devoid of CD3. After another 48 hours without the antibody, the normal array of surface molecules including CD3 appear again on all T cells. The selective removal of CD3 by internalization is thought to be the key mechanism of action of the antibody. Comodulation of the antigen receptor with CD3 explains the immunoblocking action of the antibody in vivo. The antibody is ineffective clinically if the CD3 target molecule is not modulated. The antibody is effective for induction of immunosuppression and for treating initial and steroid-resistant rejections.

Aufreinigung: Protein A or G affinity column

Reinheit: >95 % by reducing SDS-PAGE

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: > 3 mg/mL

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short time 2 to 8°C as supplied. Long time -20°C to -70°C as supplied.

Details zu CD3 epsilon

Target: CD3 epsilon (CD3E)

Andere Bezeichnung: CD3e

Pathways: T-Zell Rezeptor Signalweg, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway