The antibody BRA4F1 is directed against human CD15. The antibody does not cross react with the sialylated form of CD15 (CD15s).
Spezifität
The antibody BRA4F1 is directed against human CD15. The antibody does not cross react with the sialylated form of CD15 (CD15s).
Produktmerkmale
CD15 is a carbohydrate that is referred to as the Lewis X antigen, also known as alpha-(1,3)-fucosyltransferase 4, which is expressed on a variety of glycolipids and glycoproteins found on the cell surface of neutrophils, monocytes, eosinophils and non-hematopoietic tissues including embryonic tissues. CD15 is reported to function as an adhesion molecule and is involved in chemotaxis and phagocytosis. Expression of CD15 is also associated with a wide variety of tumour cells including some adenocarcinomas and certain types of myeloid and lymphoid leukaemias. Reed-Sternberg cells, found in cases of Hodgkins Lymphoma, also express CD15 and antibodies recognising the CD15 antigen are often used to help differentiate between Hodgkins and non-Hodgkins lymphoma.
Aufreinigung
Purified
Immunogen
BRA4F1 is a mouse IgM monoclonal antibody derived from the fusion of SP2.0-Ag14 mouse myeloma cells with splenocytes from a BALB/c mouse immunized with K562 cells.
FUT4
Reaktivität: Human
FACS
Wirt: Maus
Monoclonal
MEM-158
APC
Applikationshinweise
The BRA4F1 antibody is suitable for the detection of CD15 by flow cytometry, immunocytochemisty and immunohistochemistry of frozen and paraffin embedded tissues. Optimal antibody dilutions for the different applications should be determined by titration.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
Each vial contains 100 μL 1 mg/mL purified monoclonal antibody in PBS containing 0.09 % sodium azide. The product is delivered in lyophilised format.
Buffer
Each vial contains 100 μL 1 mg/mL purified monoclonal antibody in PBS containing 0.09 % sodium azide. The product is delivered in lyophilised format.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.