HMGB1 Antikörper

Produktnummer ABIN7670629

Dieses Anti-HMGB1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von HMGB1 in WB und ELISA. Geeignet für Human, Maus und Ratte.

Kurzübersicht für HMGB1 Antikörper (ABIN7670629)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HMGB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-HMGB1 Antikörper

Verwendungszweck: HMGB-1 Polyclonal Antibody(Capture/Detector)

Aufreinigung: Antigen Affinity Purification

Immunogen: Recombinant Human HMGB-1 protein expressed by E.coli

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:500-1:1000,ELISA Capture 2-8 μg/mL,ELISA Detector 0.1-0.4 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Sterile PBS , pH 7.4

Konservierungsmittel: Without preservative

Handhabung: Avoid freeze / thaw cycles. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C Valid for 1 month or -20°C Valid for 12 months

Haltbarkeit: 12 months

Details zu HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Andere Bezeichnung: HMGB-1

Hintergrund: HMGB1,HMG1,High mobiLity group protein B1,High mobiLity group protein 1 (HMG-1),Human High-mobility group box 1 protein (HMGB1),previously known as HMG-1 or amphoterin,is a member of the high mobility group box family of non-histone chromosomal proteins.HMGB1 is expressed at high levels in almost all cells. It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins. It is now known that HMGB1 can also act extracellularly,both as an inflammatory mediator that promotes monocyte migration and cytokine secretion,and as a mediator of T cell-dendritic cell interaction. The cytokine activity of HBMG1 is restricted to the HMG B box,while the A box is associated with the helix-loop-helix domain of transcription factors. HMBG1 is released in response to cell death and as a secretion product. Although HMBG-1 does not possess a classic signal sequence,it appears to be secreted as an acetylated form via secretory endolysosome exocytosis. Once secreted,HMGB1 transduces cellular signals through its high affinity receptor,RAGE and,possibly,TlR2 and TlR4. Human HMGB1 is 100 % aa identical to canine HMGB1 and 99 % aa identical to mouse,rat,bovine and porcine HMGB1,respectively.

Molekulargewicht:

Calculated MW: 29kDa

ObservedMW: 29kDa

UniProt: P09429

Pathways: p53 Signalweg, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome