XRCC3 Antikörper

Produktnummer ABIN7662061

Dieses Maus Monoklonal-Antikörper erkennt spezifisch XRCC3 in . Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für XRCC3 Antikörper (ABIN7662061)

Target: XRCC3 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 3 (XRCC3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser XRCC3 Antikörper ist unkonjugiert

Applikation: Bitte anfragen

Klon: 10F1-6

Produktdetails anti-XRCC3 Antikörper

Verwendungszweck: XRCC3(10F1/6)

Produktmerkmale: The x-ray repair cross-complementing (XRCC) proteins are responsible for efficiently repairing and maintaining genetic stability following DNA base damage. These genes share sequence similarity with the yeast DNA repair protein Rad51. XRCC1 is a protein that facilitates the DNA base excision repair pathway by interacting with DNA ligase III and DNA polymerase to repair DNA single-strand breaks. XRCC2 and XRCC3 are both involved in maintaining chromosome stability during cell division. XRCC2 is required for efficient repair of DNA double-strand breaks by homologous recombination between sister chromatids, and XRCC3 interacts directly with Rad51 to cooperate with Rad51 during recombinational repair. XRCC4 is an accessory factor of DNA Ligase IV that preferentially binds DNA with nicks or broken ends. XRCC4 binds to DNA Ligase IV and enhances its joining activity, and it is also involved in V(D)J recombination. Any defect in one of the known components of the DNA repair/V(D)J recombination machinery (Ku-70, Ku-80, DNA-PKCS, XRCC4 and DNA Ligase IV) leads to abortion of the V(D)J rearrangement process and early block in both T and B cell maturation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human XRCC3 protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Kommentare:

Positive Control: Human placenta. HeLa nuclear extract.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu XRCC3

Target: XRCC3 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 3 (XRCC3))

Andere Bezeichnung: XRCC3

Hintergrund:

Synonyms: CMM6, DNA repair protein XRCC3, RAD51 like, X ray repair complementing defective repair in Chinese hamster cells 3, X-ray repair cross-complementing protein 3, XRCC3

Gene Symbol: XRCC3

Molekulargewicht: 40 kDa

Gen-ID: 7517

UniProt: O43542

Pathways: DNA Reparatur