Vinculin Antikörper (AA 174-322) (CF®647)

Produktnummer ABIN7661957

Der Maus Monoklonal Anti-Vinculin-Antikörper wurde für WB, IHC und IHC (fp) validiert. Er ist geeignet, Vinculin in Proben von Human zu detektieren.

Kurzübersicht für Vinculin Antikörper (AA 174-322) (CF®647) (ABIN7661957)

Target: Vinculin (VCL)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Vinculin Antikörper ist konjugiert mit CF®647

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: VCL-2575

Produktdetails anti-Vinculin Antikörper (CF®647)

Bindungsspezifität: AA 174-322

Verwendungszweck: Vinculin (Marker of Age-related Macular Degeneration)(VCL/2575), CF647 conjugate

Produktmerkmale: Focal adhesions are identified as areas within the plasma membrane of tissue culture cells that adhere tightly to the underlying substrate. In vivo, these regions are involved in the adhesion of cells to the extracellular matrix. Paxillin and vinculin are cytoskeletal, focal adhesion proteins that are components of a protein complex which links the Actin network to the plasma membrane. Vinculin binding sites have been identified on other cytoskeletal proteins, including talin and alpha-actinin each contain actin binding sites. Reportedly, vinculin is a potential plasma biomarker for Age-related Macular Degeneration (AMD). The early detection of AMD using novel plasma biomarkers with genetic modeling may enable timely treatment and vision preservation in the elderly. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 174-322) of human Vinculin (VCL) protein

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Positive Control: A431, K562, NIH3T3, U-87, THP-1 cells. Human Bladder and Testis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Vinculin

Target: Vinculin (VCL)

Andere Bezeichnung: Vinculin

Hintergrund:

Synonyms: CMD1W, CMH15, Epididymis luminal protein 114, HEL114, Metavinculin, MV, MVCL, VCL, VINC

Gene Symbol: VCL

Molekulargewicht: 117 kDa

Gen-ID: 7414

UniProt: P18206

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2