VLDLR Antikörper (C-Term) (CF®740)

Produktnummer ABIN7661870

Der Maus Monoklonal Anti-VLDLR-Antikörper wurde für IHC und IHC (fp) validiert. Er ist geeignet, VLDLR in Proben von Human und Ratte zu detektieren.

Kurzübersicht für VLDLR Antikörper (C-Term) (CF®740) (ABIN7661870)

Target: VLDLR (Very Low Density Lipoprotein Receptor (VLDLR))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser VLDLR Antikörper ist konjugiert mit CF®740

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: VLDLR-1337

Produktdetails anti-VLDLR Antikörper (CF®740)

Bindungsspezifität: C-Term

Verwendungszweck: VLDL-Receptor (Very Low Density Lipoprotein Receptor) (VLDLR/1337), CF740 conjugate

Produktmerkmale: VLDLR (very low density lipoprotein receptor) is a member of the LDL receptor gene family, which includes LDL receptor, LRP, megalin, VLDLR and ApoER2. The LDL receptor family is characterized by a cluster of cysteine-rich class A repeats, epidermal growth factor (EGF)-like repeats, YWTD repeats and an O-linked sugar domain. VLDLR associates with RAP (receptor associated protein) during receptor folding, and RAP facilitates the secretion of the extracellular region of VLDLR. VLDLR is thought to mediate the interaction of extracellular Reelin and cytosolic mDab1 (mammalian disabled protein), which activates a tyrosine kinase. This pathway regulates the migration of neurons along the radial glial fiber network during brain development.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human VLDLR fragment from c-terminal

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (formalin) 1-2 μg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: HeLa or U-251 cells. Pancreas, Cerebellum, Heart or Skeletal Muscle.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu VLDLR

Target: VLDLR (Very Low Density Lipoprotein Receptor (VLDLR))

Andere Bezeichnung: VLDL Receptor

Hintergrund:

Synonyms: Very low-density lipoprotein receptor, VLDL receptor, VLDL-R, VLDLR

Gene Symbol: VLDLR

Molekulargewicht: 143-161 kDa

Gen-ID: 7436

Pathways: Cellular Response to Molecule of Bacterial Origin