ERBB4 Antikörper (AA 1116-1269)

Produktnummer ABIN7661754

Dieses Anti-ERBB4-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von ERBB4 in IF und FACS. Geeignet für Human und Maus.

Kurzübersicht für ERBB4 Antikörper (AA 1116-1269) (ABIN7661754)

Target: ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ERBB4 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: HFR-1

Produktdetails anti-ERBB4 Antikörper

Bindungsspezifität: AA 1116-1269

Verwendungszweck: HER-4 / ERBB4(HFR-1)

Produktmerkmale: The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. The gene encoding ErbB-4 is expressed as a full-length protein, which produces a short membrane-anchored cytoplasmic domain fragment and a long ectodomain fragment. The short fragment is heavily tyrosine phosphorylated and possesses tyrosine kinase catalytic activity toward an exogenous substrate. Proteolytic cleavage of ErbB-4 is promoted by the binding of heregulin. ErbB-4 is involved in cell proliferation and differentiation and its expression is highest in breast carcinoma cell lines, normal skeletal muscle, heart, pituitary, brain and cerebellum. Its expression in breast cancer, pediatric brain cancer and other types of carcinomas has been reported in studies which suggest ErbB4 expression is involved in both normal tissue development and carcinogenesis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human ERBB4 (HER4) protein fragment (around aa 1116-1269)

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: Predicted to react with Rat, Chicken, Flow cytometry: 1-2 μg/million cells, Immunofluorescence: 1-2 μg/mL, Optimal dilution for a specific application should be determined.

Kommentare:

Positive Control: Breast, brain, heart or kidney. MCF-7 cells.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, no BSA, no azide

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at -20 °C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu ERBB4

Target: ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))

Andere Bezeichnung: ERBB4

Hintergrund:

Synonyms: Avian erythroblastic leukemia viral oncogene homolog 4, ERBB4, HER4, human epidermal growth factor receptor 4, p180erbB4, Proto-oncogene-like protein c-ErbB-4

Gene Symbol: ERBB4

Molekulargewicht: 180 kDa (precursor), 80/120 kDa (cleaved)

Gen-ID: 2066

UniProt: Q15303

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung