Rekombinanter Ubiquitin Antikörper (AA 1-119) (CF®647)

Produktnummer ABIN7661576

Der Kaninchen Monoklonal Anti-Ubiquitin-Antikörper wurde für IHC und IHC (fp) validiert. Er ist geeignet, Ubiquitin in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter Ubiquitin Antikörper (AA 1-119) (CF®647) (ABIN7661576)

Target: Ubiquitin (Ub)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Ubiquitin Antikörper ist konjugiert mit CF®647

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: UBB-3143R

Produktdetails anti-Ubiquitin Antikörper (CF®647)

Bindungsspezifität: AA 1-119

Verwendungszweck: Ubiquitin (Autophagy Marker)(UBB/3143R), CF647 conjugate

Produktmerkmale: Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e. g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human Ubiquitin protein (around aa 1-119)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Positive Control: HeLa or Jurkat cells. Alzheimer Brain.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Ubiquitin

Target: Ubiquitin (Ub)

Andere Bezeichnung: Ubiquitin

Hintergrund:

Synonyms: Epididymis secretory protein Li 50, Polyubiquitin B, RPS27A, UBA52, UBA80, UBB, UBC, UBCEP1, UBCEP2

Gene Symbol: UBB

Molekulargewicht: 9 kDa

Gen-ID: 7314

UniProt: P62979, P62987, P62988

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway