Ubiquitin Antikörper (AA 1-119) (CF®405S)

Produktnummer ABIN7661569

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Ubiquitin in WB, IHC und IHC (fp). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Ubiquitin Antikörper (AA 1-119) (CF®405S) (ABIN7661569)

Target: Ubiquitin (Ub)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Ubiquitin Antikörper ist konjugiert mit CF®405S

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: UBB-2122

Produktdetails anti-Ubiquitin Antikörper (CF®405S)

Bindungsspezifität: AA 1-119

Verwendungszweck: Ubiquitin (Autophagy Marker) (UBB/2122), CF405S conjugate

Produktmerkmale: Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human Ubiquitin protein (around aa 1-119)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. ELISA For coating, order Ab without BSA. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: HeLa or Jurkat cells. Alzheimer s Brain

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Ubiquitin

Target: Ubiquitin (Ub)

Andere Bezeichnung: Ubiquitin

Hintergrund:

Synonyms: Epididymis secretory protein Li 50, Polyubiquitin B, RPS27A, UBA52, UBA80, UBB, UBC, UBCEP1, UBCEP2, Ubiquitin, Ubiquitin B

Gene Symbol: UBB

Molekulargewicht: 9 kDa

Gen-ID: 7314

UniProt: P0CG47, P0CG48, P62979, P62987, P62988

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway