SOX4 Antikörper (CF®594)

Produktnummer ABIN7660318

Dieses Maus Monoklonal-Antikörper erkennt spezifisch SOX4 in IHC und IHC (fp). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für SOX4 Antikörper (CF®594) (ABIN7660318)

Target: SOX4 (SRY (Sex Determining Region Y)-Box 4 (SOX4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SOX4 Antikörper ist konjugiert mit CF®594

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: SOX4-2540

Produktdetails anti-SOX4 Antikörper (CF®594)

Verwendungszweck: SOX4 (Master Regulator of Epithelial-Mesenchymal Transition) (SOX4/2540), CF594 conjugate

Produktmerkmale: SOX4 is a member of the SOX (SRY-related HMG-box) family of transcription factors with a critical role in embryonic development and in cell-fate determination during organogenesis of the heart, pancreas, brain, and in B and T lymphocyte differentiation. SOX4 gene expression is upregulated in many cancer types, and increased SOX4 activity contributes to cellular transformation, cell survival, and metastasis. Gene expression profiling has uncovered SOX4 with upregulated activity during TGF-β-induced epithelial-mesenchymal transition (EMT) in normal and cancerous breast epithelial cells. SOX4 is indispensable for EMT and cell survival in vitro and for primary tumor growth and metastasis in vivo. SOX4 is identified as a master regulator of EMT by governing the expression of the epigenetic modifier Ezh2.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human SOX4 protein

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Breast or Colon Carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu SOX4

Target: SOX4 (SRY (Sex Determining Region Y)-Box 4 (SOX4))

Andere Bezeichnung: SOX4

Hintergrund:

Synonyms: SOX, SRY (sex determining region Y) box 4, SRY box containing gene 4, SRY related HMG box gene 4, Transcription factor SOX-4

Gene Symbol: SOX4

Molekulargewicht: 47 kDa

Gen-ID: 6659

UniProt: Q06945