Rekombinanter SOX10 Antikörper (AA 115-269) (CF®640R)

Produktnummer ABIN7660249

Dieses Maus Monoklonal-Antikörper erkennt spezifisch SOX10 in WB, IHC und IHC (fp). Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für Rekombinanter SOX10 Antikörper (AA 115-269) (CF®640R) (ABIN7660249)

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SOX10 Antikörper ist konjugiert mit CF®640R

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: RSOX10-1074

Produktdetails anti-SOX10 Antikörper (CF®640R)

Bindungsspezifität: AA 115-269

Verwendungszweck: SOX10 (Melanoma Marker) (rSOX10/1074), CF640R conjugate

Produktmerkmale: SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human SOX10 protein fragment (around aa115-269)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: HepG2 cells. Melanomas, breast carcinomas, gliomas.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu SOX10

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Andere Bezeichnung: SOX10

Hintergrund:

Synonyms: DOM, PCWH, SOX10, SRY (sex determining region Y) box 10, SRY box containing gene 10, SRY related HMG box gene 10, Waardenburg syndrome type 2E (WS2E), WS4, Waardenburg syndrome type 4C (WS4C)

Gene Symbol: SOX10

Molekulargewicht: 49-58 kDa

Gen-ID: 6663

UniProt: P56693

Pathways: Chromatin Binding