SOX10 Antikörper (AA 115-269)

Produktnummer ABIN6940624

Der Maus Monoklonal anti-SOX10 Antikörper (Klon SOX10-992) (ABIN6940624) detektiert spezifisch SOX10 in WB und ELISA. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für SOX10 Antikörper (AA 115-269) (ABIN6940624)

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SOX10 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Klon: SOX10-992

Produktdetails anti-SOX10 Antikörper

Bindungsspezifität: AA 115-269

Verwendungszweck: SOX10 (Melanoma Marker) Antibody

Spezifität: The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a protein of ~55 kDa, identified as SOX10. This MAb is highly specific and does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled, and desmoplastic subtypes. It is expressed by metastatic melanomas and nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45 and anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes, and myoepithelial cells of salivary, bronchial and mammary glands. SOX-10 expression is also observed in mast cells.

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human SOX10 protein fragment (aa115-269)

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: HepG2 cells. Melanomas, breast carcinomas, gliomas.

Known Application: ELISA (For coating, purchase antibody without BSA), Western Blot (0.5-1.0 μg/mL)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu SOX10

Target: SOX10 (SRY (Sex Determining Region Y)-Box 10 (SOX10))

Andere Bezeichnung: SOX10

Hintergrund:

DOM, PCWH, SOX10, SRY (sex determining region Y) box 10, SRY box containing gene 10, SRY related HMG box gene 10, Waardenburg syndrome type 2E(WS2E), WS4, Waardenburg syndrome type 4C (WS4C)

Breast Carcinomas, COLO-38 or HepG2 cells. Melanomas, gliomas.

Molekulargewicht: 49-58kDa

Gen-ID: 6663, 376984

UniProt: P56693

Pathways: Chromatin Binding