Rekombinanter SPTA1 Antikörper (AA 356-475) (CF®405S)

Produktnummer ABIN7660094

Der Kaninchen Monoklonal Anti-SPTA1-Antikörper wurde für WB, IHC und IHC (fp) validiert. Er ist geeignet, SPTA1 in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter SPTA1 Antikörper (AA 356-475) (CF®405S) (ABIN7660094)

Target: SPTA1 (Spectrin alpha 1, Erythrocytic (SPTA1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser SPTA1 Antikörper ist konjugiert mit CF®405S

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: SPTA1-2939R

Produktdetails anti-SPTA1 Antikörper (CF®405S)

Bindungsspezifität: AA 356-475

Verwendungszweck: Spectrin Alpha 1 (Erythrocyte Marker) (SPTA1/2939R), CF405S conjugate

Produktmerkmale: Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. Spectrins function as membrane organizers and stabilizers, composed of non-homologous alpha and beta chains, which aggregate side-to-side in an anti-parallel fashion to form dimers, tetramers and higher polymers. Spectrin alpha I and spectrin beta I are present in erythrocytes, whereas spectrin alpha II (also designated fodrin alpha) and spectrin beta II (also designated fodrin beta) are present in other somatic cells. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human SPTA (around aa356-475)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. ELISA: 2-4 μg/m for coating order Ab without BSA. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Jurkat cells. Placenta or Tonsil.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu SPTA1

Target: SPTA1 (Spectrin alpha 1, Erythrocytic (SPTA1))

Andere Bezeichnung: Spectrin Alpha 1

Hintergrund:

Synonyms: Alpha I spectrin, Elliptocytosis 2 (EL2), Erythrocyte alpha spectrin, Erythroid alpha-spectrin, HPP, HS3, Spectrin alpha erythrocytic 1, SPH3, SPTA1

Gene Symbol: SPTA1

Tissue Expression: Red blood cells

Molekulargewicht: 240 kDa

Gen-ID: 6708

UniProt: P02549

Pathways: Regulation of Actin Filament Polymerization