Spastin Antikörper (CF®405S)

Produktnummer ABIN7660072

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Spastin in WB. Er zeigt eine Reaktivität gegenüber Maus, Ratte und Human.

Kurzübersicht für Spastin Antikörper (CF®405S) (ABIN7660072)

Target: Spastin (SPAST)

Reaktivität: Maus, Ratte, Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Spastin Antikörper ist konjugiert mit CF®405S

Applikation: Western Blotting (WB)

Klon: Sp 6C6

Produktdetails anti-Spastin Antikörper (CF®405S)

Verwendungszweck: Spastin(Sp 6C6), CF405S conjugate

Produktmerkmale: The AAA protein family members share an ATPase domain and have roles in various cellular processes including intracellular motility, membrane trafficking, proteolysis, protein folding and organelle biogenesis. Spastin, a member of the AAA protein family, is a 616 amino acid protein and is involved in the function or assembly of nuclear protein complexes. The Spastin protein is expressed ubiquitously and localizes to the nucleus and the cytoplasm, where it may also be involved in microtubule dynamics. Mutations in the Spastin gene (SPAST, SPG4) cause the most common form of spastic paraplegia 4, an autosomal dominant form of hereditary spastic paraplegia (HSP). HSPs comprise a group of inherited neurological disorders characterized by spastic lower extremity weakness due to a length-dependent, retrograde axonopathy of corticospinal motor neurons. SPAST-specific mutations account for approximately 40 % of all autosomal dominant HSPs. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human Spastin protein.

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL, Western blot 1-2 μg/mL, Optimal dilution for a specific application should be determined.

Kommentare:

Positive Control: HeLa cells. K-562 or Raji cell lysates.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Spastin

Target: Spastin (SPAST)

Andere Bezeichnung: Spastin

Hintergrund:

Synonyms: ADPSP, FSP2, Spast, Spatin, SPG4

Gene Symbol: SPAST

Molekulargewicht: 52 kDa

Gen-ID: 6683

Pathways: Microtubule Dynamics, M Phase, Regulation of Cell Size