Rekombinanter RPA2 Antikörper (CF®594)

Produktnummer ABIN7659560

Der Kaninchen Monoklonal Anti-RPA2-Antikörper wurde für WB validiert. Er ist geeignet, RPA2 in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter RPA2 Antikörper (CF®594) (ABIN7659560)

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser RPA2 Antikörper ist konjugiert mit CF®594

Applikation: Western Blotting (WB)

Klon: RPA2-3140R

Produktdetails anti-RPA2 Antikörper (CF®594)

Verwendungszweck: Replication Protein A2 (RPA2)(RPA2/3140R), CF594 conjugate

Produktmerkmale: Recognizes a protein of 34 kDa, identified as a subunit of Replication Protein A (RPA) (also known as human single-stranded DNA binding protein, or HSSB). RPA from human cells is a stable heterotrimer of 70 kDa, 32-34 kDa, and 11-14 kDa subunits (RPA70, RPA32, and RPA14 respectively). It is involved in DNA replication, repair, and recombination. RPA is required for the SV40 large tumor antigen-catalyzed unwinding of SV40 DNA and stimulates DNA polymerase (pol) alpha and delta. RPA34 is phosphorylated at the G1/S boundary of the cell cycle or upon exposure of cells to DNA damage-inducing agents including ionizing and UV radiation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length Replication protein A2 (RPA2) protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot 1-2 μg/mL, Optimal dilution for a specific application should be determined.

Kommentare:

Positive Control: MCF-7, HeLa or HepG2 cell lysates (WB). Human tonsil (IHC).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu RPA2

Target: RPA2 (Replication Protein A2, 32kDa (RPA2))

Andere Bezeichnung: Replication Protein A2

Hintergrund:

Synonyms: p32, p34, REPA2, Replication protein A 32 kDa subunit, Replication protein A 34 kDa subunit, Replication protein A2, RF-A protein 2, Rf-A2, RFA, RP-A p32, RP-A p34, RP21C, RPA32, RPA34, RpLP1, RpP2

Gene Symbol: RPA2

Molekulargewicht: 32-34 kDa

Gen-ID: 6118

UniProt: P15927

Pathways: Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA