Melanoma gp100 Antikörper (CF®740)

Produktnummer ABIN7658897

Der Maus Monoklonal Anti-Melanoma gp100-Antikörper wurde für IHC, IF, FACS, ELISA, IHC (fp) und IP validiert. Er ist geeignet, Melanoma gp100 in Proben von Human und Pferd zu detektieren.

Kurzübersicht für Melanoma gp100 Antikörper (CF®740) (ABIN7658897)

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Reaktivität: Human, Pferd

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Melanoma gp100 Antikörper ist konjugiert mit CF®740

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP)

Klon: NKI-beteb

Produktdetails anti-Melanoma gp100 Antikörper (CF®740)

Verwendungszweck: Pmel17 / gp100 / SILV(NKI-beteb), CF740 conjugate

Produktmerkmale: By immunohistochemistry, this antibody specifically recognizes a protein in melanocytes and melanomas. It reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. This Mab labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Membranes from a human melanoma metastasis

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 1-2 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunofluorescence 1-2 μg/mL. Does not react with rat, others not tested. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: SK-MEL-28 cells or Melanoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Melanoma gp100

Target: Melanoma gp100 (PMEL) (Premelanosome Protein (PMEL))

Andere Bezeichnung: gp100

Hintergrund:

Synonyms: 95 kDa melanocyte-specific secreted glycoprotein, M-beta, Melanocyte lineage specific antigen GP100, Melanocyte protein Pmel 17, Melanoma associated ME20 antigen, Melanosomal matrix protein17, p100, p26, PMEL17, Premelanosome protein, Secreted melanoma-associated ME20 antigen, SILV, Silver homolog

Gene Symbol: PMEL

Tissue Expression: Melanocytes

Molekulargewicht: 90-100 kDa

Gen-ID: 6490

UniProt: P40967