Rekombinanter Phosphotyrosine Antikörper (Phosphospecific)

Produktnummer ABIN7658616

Der Kaninchen Monoklonal anti-Phosphotyrosine Antikörper (Klon PY2870R) (ABIN7658616) detektiert spezifisch Phosphotyrosine. Dieser Antikörper reagiert spezifisch mit Proben aus Diverse Spezies.

Kurzübersicht für Rekombinanter Phosphotyrosine Antikörper (Phosphospecific) (ABIN7658616)

Target: Phosphotyrosine

Antikörpertyp: Recombinant Antibody

Reaktivität: Diverse Spezies

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Phosphotyrosine Antikörper ist unkonjugiert

Applikation: Bitte anfragen

Klon: PY2870R

Produktdetails anti-Phosphotyrosine Antikörper

Bindungsspezifität: Phosphospecific

Verwendungszweck: Phosphotyrosine (P-Tyr)(PY2870R)

Produktmerkmale: Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human phosphotyrosine protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Kommentare:

Positive Control: MCF-7, MDA-231, T47-D cells. Breast carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, no BSA, no azide

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at -20 °C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Phosphotyrosine

Target: Phosphotyrosine

Substanzklasse: Amino Acid

Hintergrund: P-Tyr

Molekulargewicht: Depends upon the phosphorylated target