Peroxiredoxin 4 Antikörper (CF®740)

Produktnummer ABIN7658579

Der Maus Monoklonal Anti-Peroxiredoxin 4-Antikörper wurde für validiert. Er ist geeignet, Peroxiredoxin 4 in Proben von Human zu detektieren.

Kurzübersicht für Peroxiredoxin 4 Antikörper (CF®740) (ABIN7658579)

Target: Peroxiredoxin 4 (PRDX4)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Peroxiredoxin 4 Antikörper ist konjugiert mit CF®740

Applikation: Bitte anfragen

Klon: CPTC-PRDX4-2

Produktdetails anti-Peroxiredoxin 4 Antikörper (CF®740)

Verwendungszweck: Peroxiredoxin 4 (Prognostic Marker for Lung SqCC)(CPTC-PRDX4-2), CF740 conjugate

Produktmerkmale: The peroxiredoxin (PRX) family comprises six antioxidant proteins, PRX I, II, III, IV, V and VI, which protect cells from reactive oxygen species (ROS) by preventing the metal-catalyzed oxidation of enzymes. The PRX proteins primarily utilize thioredoxin as the electron donor for antioxidation, although they are fairly promiscuous with regard to the hydroperoxide substrate. In addition to protection from ROS, peroxiredoxins are also involved in cell proliferation, differentiation and gene expression. PRX I, II, IV and VI show diffuse cytoplasmic localization, while PRX III and V exhibit distinct mitochondrial localization. The human PRX IV gene is expressed in many tissues. It exists as a precursor protein, which is only detected in testis, and aprocessed secreted form. PRX IV is highly expressed in lung cancer and is necessary for the promotion of lung cancer in vitro. Studies have demonstrated that PRX IV positive expression is significantly correlated with recurrences and shorter disease-free survival in patients with early-stage lung squamous cell carcinoma, and therefore can be used as a prognostic marker in lung squamous cell carcinoma. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human PRDX4 protein

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Kommentare:

Positive Control: HepG2, HeLa and Jurkat cell lysates. Ovarian carcinoma tissue.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Peroxiredoxin 4

Target: Peroxiredoxin 4 (PRDX4)

Andere Bezeichnung: Peroxiredoxin 4

Hintergrund:

Synonyms: Antioxidant enzyme AOE372, EC 1.11.1.15, Thioredoxin dependent peroxide reductase A0372, TRANK

Gene Symbol: PRDX4

Molekulargewicht: 27 kDa

Gen-ID: 10549

UniProt: Q13162