Rekombinanter ADRP Antikörper (AA 249-376)

Produktnummer ABIN7658571

Dieses Anti-ADRP-Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von ADRP in WB, IHC und IHC (fp). Geeignet für Human.

Kurzübersicht für Rekombinanter ADRP Antikörper (AA 249-376) (ABIN7658571)

Target: ADRP (PLIN2) (Perilipin 2 (PLIN2))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser ADRP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: ADFP-2755R

Produktdetails anti-ADRP Antikörper

Bindungsspezifität: AA 249-376

Verwendungszweck: Adipophilin / Perilipin-2 (Marker of Lipid Accumulation) (ADFP/2755R)

Produktmerkmale: Perilipin-2, also known as Adipophilin, belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This 48 kDa protein is associated with the lipid globule surface membrane material, and maybe involved in development and maintenance of adipose tissue. However, it is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 249-376) of human Adipophilin (ADFP) protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Western blotting 1-2 μg/mL. ELISA For coating, order Ab without BSA. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: HepG2 or JAR cells. Liver, Adrenal gland or Cerebellum.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu ADRP

Target: ADRP (PLIN2) (Perilipin 2 (PLIN2))

Andere Bezeichnung: Adipophilin

Hintergrund:

Synonyms: Perilipin-2, Adipophilin, ADFP, ADRP, Adipose differentiation-related protein, Perilipin-2

Gene Symbol: PLIN2

Molekulargewicht: 48 kDa

Gen-ID: 123

UniProt: Q99541

Pathways: Regulation of Lipid Metabolism by PPARalpha, Lipid Metabolism