Rekombinanter PAX5 Antikörper (CF®647)

Produktnummer ABIN7658298

Dieses Anti-PAX5-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von PAX5 in WB. Geeignet für Human.

Kurzübersicht für Rekombinanter PAX5 Antikörper (CF®647) (ABIN7658298)

Target: PAX5 (Paired Box 5 (PAX5))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PAX5 Antikörper ist konjugiert mit CF®647

Applikation: Western Blotting (WB)

Klon: RPAX5-2060

Produktdetails anti-PAX5 Antikörper (CF®647)

Verwendungszweck: PAX5 / BSAP (Early B-Cell Marker)(rPAX5/2060), CF647 conjugate

Produktmerkmale: The specificity of this monoclonal antibody to its intended target was validated by HuProt™, Array, containing more than 19, 000, full-length human proteins. The PAX5 gene is a member of thepaired box (PAX)family of transcription factors. The central feature of this gene family is a novel, highly conservedDNA-binding domain, known as thepaired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute toneoplastic transformation. The PAX5 gene encodes theB-celllineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development andspermatogenesis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length humanPAX5 protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: ELISA: For coating, purchase antibody without BSA, Western blot 1-2 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

Kommentare:

Positive Control: Daudi or Ramos cells. Tonsil, spleen, Lymph Nodeor Colon Carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu PAX5

Target: PAX5 (Paired Box 5 (PAX5))

Andere Bezeichnung: BSAP

Hintergrund:

Synonyms: B cell lineage specific activator, BSAP, EBB-1, KLP, Paired box 5

Gene Symbol: PAX5

Tissue Expression: B-cells

Molekulargewicht: 42 kDa

Gen-ID: 5079

UniProt: Q02548