ERK1/2 Antikörper (pThr183, pTyr185)

Produktnummer ABIN732458

Der Kaninchen Polyklonal Anti-ERK1/2-Antikörper wurde für WB, ELISA, IHC (p), IF (cc), FACS, IF (p) und IHC (fro) validiert. Er ist geeignet, ERK1/2 in Proben von Human, Maus, Ratte und Huhn zu detektieren. Es sind 3+ Publikationen verfügbar.

Kurzübersicht für ERK1/2 Antikörper (pThr183, pTyr185) (ABIN732458)

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Reaktivität: Human, Maus, Ratte, Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ERK1/2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Flow Cytometry (FACS), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-ERK1/2 Antikörper

Bindungsspezifität: pThr183, pTyr185

Kreuzreaktivität: Huhn, Human, Maus, Ratte

Homologie: Dog,Cow,Pig,Horse,Rabbit,Guinea Pig

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human ERK2 around the phosphorylation site of Thr183/Tyr185

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-ERK1/2 Antikörper (ABIN732458)

Hirata, Katayama, Tsuji, Imura, Natsume, Sugahara, Kunieda, Nakamura, Otsuki: "Homeobox family Hoxc localization during murine palate formation." in: Congenital anomalies, Vol. 56, Issue 4, pp. 172-9, (2016) (PubMed).

Iriyama, Hatta, Takei: "Direct effect of dasatinib on signal transduction pathways associated with a rapid mobilization of cytotoxic lymphocytes." in: Cancer medicine, Vol. 5, Issue 11, pp. 3223-3234, (2016) (PubMed).

Wang, Fu, Jiang, Yu, Wang, Han, Liu, Wang: "Bone marrow mononuclear cell transplantation promotes therapeutic angiogenesis via upregulation of the VEGF-VEGFR2 signaling pathway in a rat model of vascular dementia." in: Behavioural brain research, Vol. 265, pp. 171-80, (2014) (PubMed).

Details zu ERK1/2

Target: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Andere Bezeichnung: ERK1+ERK2

Hintergrund:

Synonyms: ERK, p38, p4, p41, ERK2, ERT1, ERK-2, MAPK2, PRKM1, PRKM2, P42MAPK, p41mapk, p42-MAPK, ERK1, ERT2, ERK-1, PRKM3, P44ERK1, P44MAPK, HS44KDAP, HUMKER1A, p44-ERK1, p44-MAPK, Mitogen-activated protein kinase 1, MAP kinase 1, MAPK 1, Extracellular signal-regulated kinase 2, MAP kinase isoform p42, Mitogen-activated protein kinase 2, MAP kinase 2, MAPK 2, MAPK1

Background: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.

Gen-ID: 55945595

UniProt: P28482