NKX2-1 Antikörper (CF®740)

Produktnummer ABIN7657920

Dieses Anti-NKX2-1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von NKX2-1 in IHC und IHC (fp). Geeignet für Human, Maus und Ratte.

Kurzübersicht für NKX2-1 Antikörper (CF®740) (ABIN7657920)

Target: NKX2-1 (NK2 Homeobox 1 (NKX2-1))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NKX2-1 Antikörper ist konjugiert mit CF®740

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: 8G7G3-1 NX2-1-690

Produktdetails anti-NKX2-1 Antikörper (CF®740)

Verwendungszweck: TTF-1 / NKX2.1(8G7G3/1 + NX2.1/690), CF740 conjugate

Produktmerkmale: Recognizes a protein of 40 kDa, identified as Thyroid transcription factor-1 (TTF-1). TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates.Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Rat full length TTF-1 recombinant protein (8G7G3/1), Recombinant TTF-1 protein (NX2.1/690)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: MAD109, MLE-15, H441-4, or H345 cells. Normal thyroid or lung.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu NKX2-1

Target: NKX2-1 (NK2 Homeobox 1 (NKX2-1))

Andere Bezeichnung: NKX2.1

Hintergrund:

Synonyms: BCH|Benign chorea|BHC|Homeobox protein Nkx2.1|NK2 homeobox 1|NKX2.1|NKX2A|TEBP|Thyroid nuclear factor-1|Thyroid specific enhancer binding protein|Thyroid transcription factor-1 (TTF-1)|Tin man|TITF1|TTF-1

Gene Symbol: NKX2-1

Molekulargewicht: 40 kDa

Gen-ID: 7080

UniProt: P43699

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Glucan Metabolic Process, Feeding Behaviour