NAPSA Antikörper (AA 189-299) (CF®568)

Produktnummer ABIN7657711

Dieses Maus Monoklonal-Antikörper erkennt spezifisch NAPSA in WB, IHC und IHC (fp). Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für NAPSA Antikörper (AA 189-299) (CF®568) (ABIN7657711)

Target: NAPSA (Napsin A Aspartic Peptidase (NAPSA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser NAPSA Antikörper ist konjugiert mit CF®568

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: NAPSA-1238 NAPSA-1239

Produktdetails anti-NAPSA Antikörper (CF®568)

Bindungsspezifität: AA 189-299

Verwendungszweck: Napsin A (Lung Adenocarcinoma Marker)(NAPSA/1238 + NAPSA/1239), CF568 conjugate

Produktmerkmale: Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

Immunogen: Recombinant human Napsin-A protein fragment (aa189-299)

Isotyp: IgG, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistochemistry (formalin-fixed): 1-2 μg/mL for 30 minutes at RT. Western blot: 1-2 μg/mL for 60 minutes at RT. Flow cytometry: 0.5-1 μg/million cells. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Lung adenocarcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu NAPSA

Target: NAPSA (Napsin A Aspartic Peptidase (NAPSA))

Andere Bezeichnung: Napsin-A

Hintergrund:

Synonyms: ASP4, Aspartyl protease 4, KAP, Kidney derived aspartic protease like protein (Kdap), NAP1, NAPA, Napsa, napsin A aspartic peptidase, Pronapsin A, SNAPA

Gene Symbol: NAPSA

Tissue Expression: Kidney|Lung

Molekulargewicht: 37 kDa

Gen-ID: 9476

UniProt: O96009

Pathways: Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis