MILK FAT GLOBULIN Antikörper (CF®740)

Produktnummer ABIN7657115

Der Maus Monoklonal Anti--Antikörper wurde für IF, IHC, IHC (fp) und WB validiert. Er ist geeignet, in Proben von Human zu detektieren.

Kurzübersicht für MILK FAT GLOBULIN Antikörper (CF®740) (ABIN7657115)

Target: MILK FAT GLOBULIN

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: CF®740

Applikation: Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Western Blotting (WB)

Klon: MFG-06

Produktdetails

Verwendungszweck: Milk Fat Globulin(MFG-06), CF740 conjugate

Produktmerkmale: Recognizes a protein of 40-45 kDa, identified as human milk fat globule membrane protein (HMFG). HMFG is present on normal human breast epithelial cells and cell lines derived from breast carcinomas, as well as to the outer surface of the human milk fat globule. HMFG is considered as a differentiation marker. It is useful as specific breast epithelial marker and can also provide a tool to study the role of the cell surface in normal and neoplastic mammary development.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human milk fat globule membrane preparation

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Normal breast or breast carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Antigendetails

Target: MILK FAT GLOBULIN

Andere Bezeichnung: Milk-Fat-Globulin

Hintergrund:

Synonyms: Breast epithelial antigen BA46, EDIL1, HMFG, Lactadherin, Lactadherin precursor, Medin, MFGE8, MFGM, Milk fat globule-EGF factor 8 (MFG-E8)

Gene Symbol: MFGE8

Tissue Expression: Epithelial cells|Mammary gland

Molekulargewicht: 45 kDa

Gen-ID: 4240

UniProt: Q08431