Rekombinanter MITF Antikörper (CF®740)

Produktnummer ABIN7657109

Der Kaninchen Monoklonal Anti-MITF-Antikörper wurde für IHC und IHC (fp) validiert. Er ist geeignet, MITF in Proben von Human und Hund zu detektieren.

Kurzübersicht für Rekombinanter MITF Antikörper (CF®740) (ABIN7657109)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Hund

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser MITF Antikörper ist konjugiert mit CF®740

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: MITF-2987R

Produktdetails anti-MITF Antikörper (CF®740)

Verwendungszweck: Microphthalmia Transcription Factor (MITF)(MITF/2987R), CF740 conjugate

Produktmerkmale: MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. This MAb recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human MiTF protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Positive Control: Jurkat, A-431, HeLa or 501 Mel human melanoma cells. Melanoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Andere Bezeichnung: Microphthalmia Transcription Factor

Hintergrund:

Synonyms: BHLHE32, Class E basic helix-loop-helix protein 32 (bHLHe32), CMM8, Mi, Microphthalmia-associated transcription factor, MITF, WS2, WS2A

Gene Symbol: MITF

Tissue Expression: Melanocytes

Molekulargewicht: 52-56 kDa (doublet)

Gen-ID: 4286

UniProt: O75030

Pathways: Chromatin Binding