MITF Antikörper

Produktnummer ABIN6940049

Der Maus Monoklonal anti-MITF Antikörper (Klon MITF-915) (ABIN6940049) detektiert spezifisch MITF in IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Human und Hund.

Kurzübersicht für MITF Antikörper (ABIN6940049)

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Reaktivität: Human, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MITF Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC)

Klon: MITF-915

Produktdetails anti-MITF Antikörper

Verwendungszweck: Microphthalmia Transcription Factor (MITF) Antibody

Spezifität: MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. This MAb recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.

Keine Kreuzreaktivität: Maus, Ratte (Rattus)

Aufreinigung: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant full-length human MiTF protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise:

Positive Control: Jurkat, A-431, HeLa or 501 Mel human melanoma cells. Melanoma.

Known Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Haltbarkeit: 24 months

Details zu MITF

Target: MITF (Microphthalmia-Associated Transcription Factor (MITF))

Andere Bezeichnung: MITF

Hintergrund:

BHLHE32, Class E basic helix-loop-helix protein 32 (bHLHe32), CMM8, Mi, Microphthalmia-associated transcription factor, MITF, WS2, WS2A

A-431, HeLa or 501 Mel human melanoma cells. Melanoma., Jurkat

Molekulargewicht: 52-56kDa (doublet)

Gen-ID: 4286, 166017, 618266

UniProt: O75030

Pathways: Chromatin Binding