Mesothelin Antikörper (AA 273-407) (CF®488A)

Produktnummer ABIN7656964

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Mesothelin in IHC und IHC (fp). Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für Mesothelin Antikörper (AA 273-407) (CF®488A) (ABIN7656964)

Target: Mesothelin (MSLN)

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Mesothelin Antikörper ist konjugiert mit CF®488A

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: MSLN-2131

Produktdetails anti-Mesothelin Antikörper (CF®488A)

Bindungsspezifität: AA 273-407

Verwendungszweck: Mesothelin (Mesothelial Marker) (MSLN/2131), CF488A conjugate

Produktmerkmale: Mesothelin is a 40 kDa glycosyl-phosphatidylinositol-anchored glycoprotein cleaved from a 69 kDa precursor protein. Mesothelin immunoreactivity is high in cancers of the ovary (serous papillary, endometrioid and undifferentiated) and pancreas, with less frequent staining seen in adenocarcinomas of the endometrium, lung and stomach/esophagus. Mesothelin is one of the most sensitive markers for mesothelioma. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment (around aa 273-407) of human Mesothelin (MSLN) protein

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: CAPAN-2 or HeLa cells. Lung Mesothelioma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Mesothelin

Target: Mesothelin (MSLN)

Andere Bezeichnung: Mesothelin

Hintergrund:

Synonyms: CAK1, Megakaryocyte potentiating factor, Mesothelin, MSLN, SMR, SMRP

Gene Symbol: MSLN

Molekulargewicht: 40 kDa (Mature form), 69 kDa (Precursor)

Gen-ID: 10232

UniProt: Q13421

Pathways: EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process