MMP2 Antikörper (AA 444-575) (CF®740)

Produktnummer ABIN7656706

Dieses Maus Monoklonal-Antikörper erkennt spezifisch MMP2 in WB. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für MMP2 Antikörper (AA 444-575) (CF®740) (ABIN7656706)

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MMP2 Antikörper ist konjugiert mit CF®740

Applikation: Western Blotting (WB)

Klon: MMP2-1501

Produktdetails anti-MMP2 Antikörper (CF®740)

Bindungsspezifität: AA 444-575

Verwendungszweck: MMP2 / Collagenase Type IV A (Tumor Metastasis Marker) (MMP2/1501), CF740 conjugate

Produktmerkmale: This antibody recognizes a protein of 72 kDa, which is identified as MMP2. The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, Fibronectin, Laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-2 (also designated type IV collagenase) cleaves collagen types IV,V, VII and X and gelatin type I. Activation of MMP-2 secretion requires the Ras signaling pathway. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human MMP2 protein fragment (aa444-575)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: U-138 MG or U-87 MG cells. Placenta

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu MMP2

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Andere Bezeichnung: Collagenase-Type-IV-A

Hintergrund:

Synonyms: Type IV collagenase|CLG4A|Collagenase Type 4 alpha|Collagenase type IV A|Gelatinase A|Gelatinase alpha|Gelatinase neutrophil|Matrix metalloproteinase-2|MMP2|MONA|Neutrophil gelatinase|PEX|TBE-1

Gene Symbol: MMP2

Molekulargewicht: 72 kDa (Pro), 63 kDa (cleaved)

Gen-ID: 4313

UniProt: P08253

Pathways: Activation of Innate immune Response