HLA-B Antikörper (CF®740)

Produktnummer ABIN7656463

Dieses Maus Monoklonal-Antikörper erkennt spezifisch HLA-B in FACS und IHC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für HLA-B Antikörper (CF®740) (ABIN7656463)

Target: HLA-B (Major Histocompatibility Complex, Class I, B (HLA-B))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HLA-B Antikörper ist konjugiert mit CF®740

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC)

Klon: JOAN-1

Produktdetails anti-HLA-B Antikörper (CF®740)

Verwendungszweck: HLA-B(JOAN-1), CF740 conjugate

Produktmerkmale: HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Normal human peripheral blood lymphocytes

Isotyp: IgG3, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Human peripheral blood lymphocytes, tonsil or lymph node

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu HLA-B

Target: HLA-B (Major Histocompatibility Complex, Class I, B (HLA-B))

Andere Bezeichnung: HLA-B

Hintergrund:

Synonyms: HLA-B, HLAB, MHC Class I HLA heavy chain, Leukocyte antigen class I-B

Gene Symbol: HLA-B

Molekulargewicht: 45 kDa

Gen-ID: 3106

UniProt: P03989

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response