MHC Class II Antikörper (CF®740)

Produktnummer ABIN7656454

Dieses Maus Monoklonal-Antikörper erkennt spezifisch MHC Class II in FACS und IF. Er zeigt eine Reaktivität gegenüber Human, Rind (Kuh) und Cat.

Kurzübersicht für MHC Class II Antikörper (CF®740) (ABIN7656454)

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Reaktivität: Human, Rind (Kuh), Cat

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MHC Class II Antikörper ist konjugiert mit CF®740

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 169-1B5-2

Produktdetails anti-MHC Class II Antikörper (CF®740)

Verwendungszweck: MHC II (HLA-DRA)(169-1B5.2), CF740 conjugate

Produktmerkmale: This antibody detects a monomorphic general framework determinant of HLA-DR Class II antigen. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36 kDa alpha (heavy) chain and a 28 kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 Molecules, HLA-DR is critical for efficient peptide presentation to CD4 T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human peripheral blood leukocytes

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Ramos, Daudi or HuT78 cells. Tonsil or lymph node

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu MHC Class II

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Andere Bezeichnung: MHC-II

Hintergrund:

Synonyms: Ia3, Ia-3, H-2Ea, H2-Ea, AI323765, I-Ealpha, E-alpha-f, MHC-H2-Ea

Gene Symbol: HLA-DRA

Molekulargewicht: 36 kDa (? chain) and 27 kDa (? chain)

Gen-ID: 3122

UniProt: P01903