MHC Class II Antikörper (Biotin)

Produktnummer ABIN7505997

Dieser Ratte Monoklonal Antikörper detektiert spezifisch MHC Class II in FACS, WB, IP, IHC (fro) und IHC (p). Es zeigt Reaktivität gegenüber Proben von Maus.

Kurzübersicht für MHC Class II Antikörper (Biotin) (ABIN7505997)

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser MHC Class II Antikörper ist konjugiert mit Biotin

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: M5-114

Produktdetails anti-MHC Class II Antikörper (Biotin)

Verwendungszweck: Anti-Ms MHC Class II Biotin

Produktmerkmale: The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared extracellular determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.

Aufreinigung: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Immunogen: Activated C57BL/6 mouse spleen cells

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Details zu MHC Class II

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Andere Bezeichnung: MHC Class II

Hintergrund: MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.

Gen-ID: 35