LAG3 Antikörper

Produktnummer ABIN7656425

Der Maus Monoklonal Anti-LAG3-Antikörper wurde für validiert. Er ist geeignet, LAG3 in Proben von Human zu detektieren.

Kurzübersicht für LAG3 Antikörper (ABIN7656425)

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LAG3 Antikörper ist unkonjugiert

Applikation: Bitte anfragen

Klon: LAG3-3261

Produktdetails anti-LAG3 Antikörper

Verwendungszweck: Lymphocyte Activation Gene 3 (LAG-3) (Negative Checkpoint Regulator)(LAG3/3261)

Produktmerkmale: LAG-3 (also called CD223) is a high affinity MHC class II ligand present on the surface of CD4 CD8 T cells and NK cell, with shared homology in structure to CD4 Molecules. It has a glutamic acid-proline (EP) repetitive sequence found in other functionally distinct mammalian, parasitic, and bacterial proteins that may influence a conserved biological function. LAG-3 CD4 CD8 T cells can associate with the T cell receptor (TCR) and downregulate TCR signaling in vitro. LAG-3 inhibits CD4-dependent T cell function via its cytoplasmic domain. LAG-3 Lys-468 within a conserved 'KIEELE' motif is essential for interaction with downstream signaling molecules. Furthermore, as a checkpoint inhibitor target, it may be superior to CTLA-4 and PD-1 since both antibodies only activate effector T-cells, whereas an antagonist LAG-3 antibody can both activate T effector cells (by downregulating the LAG-3 inhibiting signal into pre-activated LAG-3 cells) and inhibit induced (i. e. antigen-specific) Treg suppressive activity. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human LAG3 protein

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Kommentare:

Positive Control: Human tonsil or Hodgkin lymphoma (IHC-P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, no BSA, no azide

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at -20 °C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu LAG3

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Andere Bezeichnung: Lymphocyte Activation Gene 3

Hintergrund:

Synonyms: CD223, FDC protein, LAG-3

Gene Symbol: LAG3

Tissue Expression: NK cells|T-cells

Molekulargewicht: 70 kDa

Gen-ID: 3902

UniProt: P18627

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints