LIG1 Antikörper (CF®740)

Produktnummer ABIN7656374

Der Maus Monoklonal Anti-LIG1-Antikörper wurde für IP, FACS und Func validiert. Er ist geeignet, LIG1 in Proben von Human, Ratte, Maus und Rind (Kuh) zu detektieren.

Kurzübersicht für LIG1 Antikörper (CF®740) (ABIN7656374)

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Reaktivität: Human, Ratte, Maus, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LIG1 Antikörper ist konjugiert mit CF®740

Applikation: Immunoprecipitation (IP), Flow Cytometry (FACS), Functional Studies (Func)

Klon: 1A9

Produktdetails anti-LIG1 Antikörper (CF®740)

Verwendungszweck: DNA Ligase 1(1A9), CF740 conjugate

Produktmerkmale: DNA Ligase I maintains the major DNA Ligase activity in proliferating cells by joining Okazaki fragments during lagging strand DNA replication. Human DNA Ligase I also has an essential role in DNA repair pathways, where it catalyzes the formation of phosphodiester bonds between adjacent 5' phosphoryl and 3' hydroxy termini at single breaks in duplex DNA molecules. In addition, DNA Ligase I plays a role in sealing nicks during excision repair. Similar to other DNA ligases, DNA Ligase I is built around a common catalytic core. Increased levels of DNA Ligase I are found in human tumors, as compared to benign tissues, as well as in peripheral blood lymphocytes. DNA Ligase I activity is altered in the chromosomal breakage deficit Bloom's syndrome (BS). Individuals with BS either have decreased levels of abnormally thermolabile DNA Ligase I or possess a dimeric form of this enzyme. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Full-length native cow LIG1 protein

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Western blot 0.5-1 μg/mL, ELISA, Immunoprecipitation, Optimal dilution for a specific application should be determined.

Kommentare:

Positive Control: MOLT-4, PC-3 or Jurkat cell lysates.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu LIG1

Target: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Andere Bezeichnung: DNA Ligase 1

Hintergrund:

Synonyms: DNA ligase 1, DNA ligase I, DNLI1_HUMAN, LIG 1, lig1, Ligase I DNA ATP dependent, MGC117397, MGC130025, Polydeoxyribonucleotide synthase [ATP] 1

Gene Symbol: LIG1

Molekulargewicht: 133 kDa

Gen-ID: 3978

UniProt: P18858

Pathways: Telomere Maintenance, DNA Reparatur, DNA Replication, Synthesis of DNA