Rekombinanter Iba1 Antikörper (AA 1-146) (CF®405S)

Produktnummer ABIN7655766

Dieses Anti-Iba1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Iba1 in IHC und IHC (fp). Geeignet für Human.

Kurzübersicht für Rekombinanter Iba1 Antikörper (AA 1-146) (CF®405S) (ABIN7655766)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Iba1 Antikörper ist konjugiert mit CF®405S

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: RAIF1-1909

Produktdetails anti-Iba1 Antikörper (CF®405S)

Bindungsspezifität: AA 1-146

Verwendungszweck: AIF1 / Iba1 (Microglia Marker) (rAIF1/1909), CF405S conjugate

Produktmerkmale: Allograft Inflammatory Factor 1 (also known as AIF1 or IBA1) is a cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. AIF1, containing two EF domains, is induced by cytokines and Interferons. In an unstimulated state, AIF1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding AIF1 resides in the tumor necrosis factor (TNF) cluster of genes, located in the region represented by the human major histocompatibility complex (MHC). Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Purified recombinant human AIF1 protein (around aa 1-146)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Lymph Node, Tonsil or Kidney

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Andere Bezeichnung: Allograft Inflammatory Factor 1

Hintergrund:

Synonyms: Allograft inflammatory factor 1, AIF1, IBA1

Gene Symbol: AIF1

Tissue Expression: Microglia|Monocytes/macrophages

Molekulargewicht: 17 kDa

Gen-ID: 199

UniProt: P55008

Pathways: Smooth Muscle Cell Migration