Rekombinanter Involucrin Antikörper

Produktnummer ABIN7655761

Dieses Kaninchen Monoklonal-Antikörper erkennt spezifisch Involucrin in IHC und IHC (fp). Er zeigt eine Reaktivität gegenüber Human, Hund, Schwein, Gorilla und Owl Monkey.

Kurzübersicht für Rekombinanter Involucrin Antikörper (ABIN7655761)

Target: Involucrin (IVL)

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Hund, Schwein, Gorilla, Owl Monkey

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Involucrin Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: IVRN-2113R

Produktdetails anti-Involucrin Antikörper

Verwendungszweck: Involucrin (Squamous Cell Terminal Differentiation Marker)(IVRN/2113R)

Produktmerkmale: Recognizes a protein of 66 kDa-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells, a doublet of ~115 kDa and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of 105 kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length Involucrin protein (IVRN)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Positive Control: MCF-7 cells. Localized to upper spinous and granular layers in normal skin.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS, 0.05 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu Involucrin

Target: Involucrin (IVL)

Andere Bezeichnung: Involucrin

Hintergrund:

Synonyms: IVL

Gene Symbol: IVL

Tissue Expression: Epidermal cells|Epithelial cells|Eye

Molekulargewicht: 66-170 kDa

Gen-ID: 3713

UniProt: P07476