IL-4 Antikörper (CF®640R)

Produktnummer ABIN7655714

Der Maus Monoklonal Anti-IL-4-Antikörper wurde für validiert. Er ist geeignet, IL-4 in Proben von Human zu detektieren.

Kurzübersicht für IL-4 Antikörper (CF®640R) (ABIN7655714)

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IL-4 Antikörper ist konjugiert mit CF®640R

Applikation: Bitte anfragen

Klon: IL4-1597

Produktdetails anti-IL-4 Antikörper (CF®640R)

Verwendungszweck: IL-4 / Interleukin 4(IL4/1597), CF640R conjugate

Produktmerkmale: Interleukin-4 (IL-4), also designated B cell stimulatory factor-1, is a glycosylated cytokine secreted by activated T lymphocytes, basophils and mast cells. The secreted IL-4 protein promotes the growth and differentiation of cells that participate in immune defense by favoring such events as the expansion of the Th2 lineage relative to Th1 cells. These T helper cell subsets are defined by their pattern of cytokine secretion: Th1 cells secrete IL-2, TNF, while Th2 cells secrete IL-4, IL-5 and IL-10. Another key immunological function of IL-4 is to induce immunoglobulin class switching. IL-4 has been shown to induce the production of IgE and enhance IgG4 secretion by B cells, but suppress the production of IgM, IgA, IgG1, IgG2 and IgG3. It has been determined that Stat6 is rapidly tyrosine phosphorylated following stimulation of IL-3 or IL-4, but is not detectably phosphorylated following stimulation with IL-2, IL-12 or erythropoietin. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human IL-4 protein

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry

Kommentare:

Positive Control: Spleen.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % BSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu IL-4

Target: IL-4 (IL4) (Interleukin 4 (IL4))

Andere Bezeichnung: IL-4

Hintergrund:

Synonyms: B cell growth factor 1, B cell IgG differentiation factor, B Cell Stimulatory Factor 1, BCGF1, Binetrakin, BSF1, IGG1 induction factor, Il4e12, Interleukin 4 variant 2, Interleukin 4, isoform 1Lymphocyte stimulatory factor 1, MGC79402, Pitrakinra

Gene Symbol: HIL41

Tissue Expression: Basophils|Mast cells|T-cells

Molekulargewicht: 18 kDa

Gen-ID: 3565

UniProt: P05112

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport, Activated T Cell Proliferation