ICAM-3/CD50 Antikörper (CF®740)

Produktnummer ABIN7655567

Der Maus Monoklonal Anti-ICAM-3/CD50-Antikörper wurde für IF, IHC und IHC (fp) validiert. Er ist geeignet, ICAM-3/CD50 in Proben von Human zu detektieren.

Kurzübersicht für ICAM-3/CD50 Antikörper (CF®740) (ABIN7655567)

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ICAM-3/CD50 Antikörper ist konjugiert mit CF®740

Applikation: Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: 186-2G9

Produktdetails anti-ICAM-3/CD50 Antikörper (CF®740)

Verwendungszweck: CD50 (ICAM-3)(186-2G9), CF740 conjugate

Produktmerkmale: Recognizes the D1 domain of an N-glycosylated glycoprotein of 120 kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3 (Leucocyte Workshop VI). CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This MAb is excellent for staining of formalin/paraffin tissues.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Stimulated human leukocytes

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Blocks binding of CD11a LFA-1 to CD50 ICAM-3. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: HL-60 or THP-1 cells. Lymph node and tonsil

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu ICAM-3/CD50

Target: ICAM-3/CD50 (ICAM3) (Intercellular Adhesion Molecule 3 (ICAM3))

Andere Bezeichnung: CD50

Hintergrund:

Synonyms: ICAMR, Intercellular adhesion molecule 3 (ICAM3)

Gene Symbol: ICAM3

Tissue Expression: Lymphocytes|T-cells

Molekulargewicht: 110-160 kDa

Gen-ID: 3385

UniProt: P32942