Rekombinanter ISR-alpha Antikörper (Extracellular Domain) (CF®740)

Produktnummer ABIN7655327

Der Kaninchen Monoklonal Anti-ISR-alpha-Antikörper wurde für IHC und IHC (fp) validiert. Er ist geeignet, ISR-alpha in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter ISR-alpha Antikörper (Extracellular Domain) (CF®740) (ABIN7655327)

Target: ISR-alpha (Insulin Receptor alpha (ISR-alpha))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser ISR-alpha Antikörper ist konjugiert mit CF®740

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Klon: INSR-2277R

Produktdetails anti-ISR-alpha Antikörper (CF®740)

Bindungsspezifität: Extracellular Domain

Verwendungszweck: Insulin Receptor Alpha (INSR/2277R), CF740 conjugate

Produktmerkmale: The insulin receptor (INSR) is a heterodimeric protein complex that has an intracellular beta subunit and an extracellular alpha subunit, which is disulfide-linked to a transmembrane segment. The insulin ligand binds to the INSR and initiates molecular signaling pathways that promote glucose uptake in cells and glycogen synthesis. Insulin binding to INSR induces phosphorylation of intra-cellular tyrosine kinase domains and recruitment of multiple SH2 and SH3 domain-containing intracellular proteins that serve as signaling intermediates for pleiotropic effects of insulin. Type 1 diabetes is an autoimmune condition of the endocrine pancreas that results in destruction of insulin secreting cells and a progressive loss in insulin-sensitive glucose uptake by cells.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of extracellular domain of human Insulin Receptor alpha

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

Kommentare:

Positive Control: Jurkat cells. Human pancreas.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: PBS, 0.1 % rBSA, 0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Lagerung: 4 °C

Informationen zur Lagerung:

Stable at room temperature or 37°C for 7 days.

Protect from light

Store at 2 to 8°C. Protect fluorescent conjugates from light

Haltbarkeit: 24 months

Details zu ISR-alpha

Target: ISR-alpha (Insulin Receptor alpha (ISR-alpha))

Andere Bezeichnung: Insulin Receptor Alpha

Hintergrund:

Synonyms: CD220, HHF5, HIR A, INSR, Insulin receptor, Insulin receptor subunit alpha, IR

Gene Symbol: INSR

Molekulargewicht: 135 kDa

Gen-ID: 3643

UniProt: P06213